Thermo Scientific™ Detoxi-Gel™ Endotoxin Removing Gel

Bind and extract endotoxins (lipopolysaccharides or LPS) from antibody or protein samples using bulk resin or columns of immobilized Polymixin B ligand.

Thermo Scientific™ Detoxi-Gel Endotoxin Removing Gel and Columns provide a quick method to remove endotoxins (lipopolysaccharides or LPS) from samples.

Detoxi-Gel Resin is a beaded agarose support onto which polymixin B has been immobilized. This ligand binds to the lipid A portion of lipopolysaccharides (LPS), which are the dominant form of endotoxins that affect biological samples. Even low levels of endotoxins can be toxic to cells or organisms and must be removed before biological samples can be added to cells for various treatment experiments. Detoxi-Gel Resin provides a quick method for removal of endotoxins from samples. It is available in convenient pre-packed columns and two sizes of slurry for use in small-scale or large-scale processes.

Highlights:

• Affinity-based – uses immobilized polymixin B to remove endotoxins by binding to their lipid A domains

• High capacity – 1mL of Detoxi-Gel Resin removes at least 9995 endotoxin units from a 5mL sample containing 10,000 EU of lipopolysaccharide (LPS)

• Reusable – no loss in binding capacity even after 10 regenerations; requires activation and regeneration with 1% sodium deoxycholate (Part No. 89904)

• Testing required – protein recovery is dependent upon protein type and concentration; empirical testing is required to validate the method for particular protein samples

• Versatile – remove endotoxin from protein solutions, cell culture media and pharmacological components

Requires:

Sodium deoxycholate (Part No. 89904) for resin activation and regeneration.

Capacity (Metric)

4000 to 6000 EU Binding per mL Gel

Content And Storage

Store at 4°C.

Product Line

Detoxi-Gel™

Quantity

1000 mL

Target

Endotoxin Removal

Type

Endotoxin Removing Gel

Stationary Phase

Polymixin B

Sufficient For

4000 to 6000 EU per mL Gel

Format

Bottle

Column Type

Affinity, Agarose Resin

For Research Use Only. Not for use in diagnostic procedures.