Thermo Scientific™ DH10B Competent Cells; High Efficiency

High efficiency, chemically competent E. coli cells. The DH10B strain is suitable for cloning DNA containing methylcytosine, 5-hydroxymethylcytosine, and methyladenine, allowing both prokaryotic and eukaryotic genomic DNA to be cloned efficiently.

• Suitable for cloning DNA containing methylcytosine, 5-hydroxymethylcytosine, and methyladenine, allowing both prokaryotic and eukaryotic genomic DNA to be cloned efficiently
• These cells are ideal for the construction of gene banks or for the generation of cDNA libraries using plasmid-derived vectors
• The φ80dlacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors to allow blue/white colony screening on bacterial agar plates containing Bluo-Gal or X-Gal
• High transformation efficiency: >1x109 cfu/µg pUC19 DNA
• Suitable for routine and high-throughput cloning applications
• Allows for blue/white colony screening
• Convenient two reactions per vial packaging
• S.O.C. Outgrowth medium included
• Suitable for variety of applications requiring high transformation efficiency:
  • Efficient DNA cloning both of prokaryotic and eukaryotic origin
  • Cloning of large plasmids and BACs
  • Ideal for generation of cDNA libraries using plasmid-derived vectors
  • Site directed mutagenesis
• Genotype: F– mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ (ara-leu)7697 galU galK λ– rpsL(StrR) nupG

Note: Optimized for use with the CloneJET PCR Cloning Kit (Cat. Nos. K1231, K1232) and Phusion Site-Directed Mutagenesis Kit (Cat. No. F541), and available as combo versions (Cat. Nos. K123120, K123240, F542)

Description

Chemically Competent

Contains F' Episome

Lacks F' Episome

Content And Storage

Dry Ice (-80°C)

Bacterial or Yeast Strain

DH10B

Species

E. coli

Format

Tube

For Use With (Application)

Cloning

Quantity

20 Reactions