Thermo Scientific™ DH5α Competent Cells; High Efficiency

High efficiency, chemically competent E. coli cells ideal for construction of gene banks or generation of cDNA libraries using plasmid-derived vectors

• The φ80dlacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors to allow blue/white colony screening on bacterial agar plates containing Bluo-Gal or X-Gal
• RecA1 and endA1 mutations in DH5α cells improve insert stability and quality of the extracted plasmid DNA
• High transformation efficiency: >1x109 cfu/µg pUC19 DNA
• Suitable for routine and high-throughput cloning applications
• Genetic markers that allow for blue/white colony screening
• Convenient two reactions per vial packaging
• S.O.C. Outgrowth medium included
• DH5α Competent Cells are suitable for variety of applications requiring high transformation efficiency:
  • Efficient DNA cloning derived from PCR, cDNA-generation reactions
  • recA1 marker facilitates working with difficult to transform DNA
  • Ideal for generation of cDNA libraries using plasmid-derived vectors
  • Site-directed mutagenesis
• Genotype: F– φ80lacZΔ M15 Δ (lacZYA-argF) U169 recA1 endA1 hsdR17 (rK– mK+) phoA supE44 λ- thi–1 gyrA96 relA1

Description

Chemically Competent

Contains F' Episome

Lacks F' Episome

Content And Storage

Dry Ice (-80°C)

Bacterial or Yeast Strain

DH5α

Species

E. coli

Format

Tube

For Use With (Application)

Cloning

Quantity

20 Reactions