Thermo Scientific™ DH5α Competent Cells

Description

Chemically competent E. coli cells recommended for routine subcloning into plasmid vectors. Subcloning efficiency cells are not suitable for the generation of cDNA libraries.

• The φ80dlacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors to allow blue/white colony screening on bacterial agar plates containing Bluo-Gal or X-Gall
• Transformation efficiency: >1x106 cfu/μg pUC19 DNA
• Suitable for routine cloning applicationsConvenient economical packaging
• Contain genetic markers that allow for blue/white colony screening
• DH5α Competent Cells for Subcloning are suitable for the standard DNA cloning applications:
  • DNA cloning following PCR or restriction digestion reactions
  • Subcloning (transferring DNA from a parent vector to a destination vector)
  • Plasmid isolation
• Genotype: F– φ80lacZΔ M15 Δ (lacZYA-argF) U169 recA1 endA1 hsdR17 (rK– mK+) phoA supE44 λ- thi–1 gyrA96 relA1
• For Research Use Only. Not for use in diagnostic procedures.

Specifications

• Description: Chemically Competent
• Contains F' Episome: Lacks F' Episome
• Content And Storage: Dry Ice (-80°C)
• Bacterial or Yeast Strain: DH5α
• Species: E. coli
• Format: Tube
• For Use With (Application): Cloning
• Quantity: 20 Reactions