Thermo Scientific™ DSS (disuccinimidyl suberate), No-Weigh™ Format

Crosslink amines to amines with disuccinimidyl suberate composed of NHS-ester groups with a membrane-permeable, mid-length spacer for intracellular protein crosslinking.

Thermo Scientific™ Pierce DSS, also called disuccinimidyl suberate, is noncleavable and membrane permeable crosslinker that contains an amine-reactive N-hydroxysuccinimide (NHS) ester at each end of an 8-carbon spacer arm.

Highlights:

• Chemical crosslinking of intracellular proteins prior to cell lysis and immunoprecipitation
• “Fix” protein interactions to allow identification of weak or transient protein interactions
• Protein crosslinking to create bioconjugates via single-step reactions
• Crosslink oriented antibodies to Protein A or G agarose
• Immobilize proteins onto amine-coated surfaces

Chemical Reactivity

Amine-Amine

Cleavable

No

Description

DSS

Molecular Weight (g/mol)

368.35

PEGylated

No

Spacer Arm Length

11.4 Å

Content And Storage

Upon receipt store desiccated at 4°C.

Cell Permeability

Yes

Shipping Condition

Ambient

Product Line

Pierce™

Labeling Method

Chemical Labeling

Crosslinker Type

Homobifunctional

Reactive Moiety

NHS Ester

Spacer

Medium (10 to 30 Å)

Form

Powder

Quantity

1 g

Solubility

DMF, DMSO

Format

Standard, Single-use

Water Soluble

No

Product Type

Crosslinker

For Research Use Only. Not for use in diagnostic procedures.