Edvotek™ Construction and Cloning of DNA Recombinant

Includes

Ultra-pure Water, DNA Fragments for Ligation, T4 DNA Ligase in Reaction Tube, Kanamycin Sulfate, Supercoiled Control DNA Samples, JM109 BactoBeads™, 0.05 M Calcium Chloride, ReadyPour™ LB Agar, Recovery Broth, Growth Medium, Tris-EDTA-Glucose Buffer (TEG), 2M NaOH, 10% SDS, RNase (DNase-free), Tris-EDTA Buffer, Acidified Potassium Acetate, Ultra-pure Water for Restriction Digests, 10X Restriction Enzyme Reaction Buffer, Dryzymes™ Rehydration Buffer, DNA Standard Marker, Supercoiled Plasmid Vector Standard, EcoRI Dryzymes™, PvuII Dryzymes™, XhoI Dryzymes™, InstaStain™ Ethidium Bromide, 10X Gel Loading Solution, 50X Electrophoresis Buffer, UltraSpec-Agarose, Screw Cap Tubes (sterile), Microtest tubes (0.5 ml), petri plates (sterile, 60 x 15mm), 1.5 ml microcentrifuge tubes, sterile inoculating loops/needles, 10ml pipets (sterile), sterile 50 ml culture tubes, petri plates (sterile, 100 x 15 mm)

To study gene structure, function, and to enhance gene expression.

 

• It is divided into five modules
• Clones are constructed by ligation of a vector and a fragment insert
• The constructs are then transformed into competent cells and the cells are grown and selected for resistance
• Plasmid DNA is then isolated from the transformants, cleaved with restriction enzymes, and analyzed by agarose gel electrophoresis
• Recommended for college level courses
• For 5 lab groups
• Time: Enzyme digestion 30 min., Gel preparation 30 min., Electrophoresis 45 min., Staining 15 min. and Destaining 20 min.

 

Unit comprises: Electrophoresis tank and power supply, automatic micropipet with tips, balance, microwave or hot plate, waterbath, large weigh boats for staining, UV transilluminator, floating racks for microtest tubes, pipet pump or bulb, 5 or 10mL pipets, laboratory glassware, metric rulers, distilled water and ice

For Use With (Equipment)

Electrophoresis apparatus

For Use With (Application)

To study gene structure, function and to enhance gene expression

Includes

Instructions, Enzymes, plasmid DNA, restriction Enzyme dilution Buffer, Enzyme grade Water, standard DNA fragments, restriction Enzyme Reaction Buffer, Gel loading solution, Agarose powder, electrophoresis Buffer, stains, calibrated pipet