Edvotek™ DNA Fingerprinting Using Restriction Enzymes

Includes

instructions, crime scene and suspect Ready-to-Load™ DNA samples, DNA Standard Marker, Dryzymes™ - Eco RI and Hind III, Enzyme Reaction Buffer, Reconstitution Buffer, Enzyme Grade Water, UltraSpec-Agarose™, 10X Gel Loading Solution, 50X Concentrated Electrophoresis Buffer, Practice Gel Loading Solution, InstaStain™ Blue Cards, FlashBlue™ DNA Stain, Disposable Pipets, & Microcentrifuge Tubes

Requires

Required: Horizontal gel electrophoresis apparatus; DC power supply; Automatic micropipets with tips; Water bathHotplate, Bunsen burner or microwave oven

Recommended: DNA visualization system (white light); Staining tray and net; 250mL flasks; Pipet pump; Hot gloves; Marking pens;Distilled or deionized water; Ice

Learn about restriction enzymes.

• The objective of this simulated forensic analysis is to develop an understanding of DNA fingerprinting by use of restriction enzymes
• Hypothetical crime scene samples are provided in addition to DNA samples, restriction enzymes and reaction buffer to digest suspect's DNA
• Digestion products are separated by agarose gel electrophoresis
• Students determine the guilty suspect through identification of the unique restriction enzyme fragmentation patterns
• Does not contain human DNA
• Some components require refrigeration, some require freezing

Order Info

Contains materials for six gels (six lanes each) with InstaStain™ Methylene Blue.

Product Type

DNA Fingerprinting Using Restriction Enzymes Classroom Kit

For Use With (Application)

For dna fingerprinting

Quantity

6 Gels

Requires

Electrophoresis apparatus; power supply; automatic pipet with tips; waterbath; balance; microwave or hot plate; visualization (white light); misc. labware; pipet pumps or bulbs; metric rulers; floating racks; photodocumentation system (optional)

Time Range

1.45 hr.

Content And Storage

Some components require refrigeration and some require freezing