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Edvotek™ Ion-Exchange Chromatography Kit
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Includes
Materials for six separations: ion exchanger, CM-Cellulose; concentrated Potassium Acetate buffer, pH 6.0; dye mixture: blue dye, Cytochrome c; Cytochrome c Standard (10mg/mL); chromatography columns; plastic pipets; 1mL pipet; 15mL conical tubes; microcentrifuge tubes; complete instructions; background information; and study questions
Requires
5mL test tubes; ring stand with clamps for columns; 100 or 200mL beakers or flasks; 100, 250 and 500mL graduated cylinders; distilled water; 5mL pipets and pumps; and spectrophotometer and cuvets (optional, must be able to read 1mL samples)
Most biological molecules have a net charge within a pH range of 2 to 10. When the pH is altered, the net charge on molecules can change drastically.
- Learn the principles of ion-exchange chromatography by separating two charged molecules using a two-step salt gradient
- Proteins are absorbed onto a charged solid support ion-exchange column and subsequently separated during elution under conditions that influence their net charge
Specifications
Product Type
Ion Exchange Chromatography Kit
For Use With (Application)
Learn The Principles of Ion-Exchange Chromatography
Includes
Instructions, ion exchanger, chemical mixture, potassium acetate buffer, chromatography columns
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