Edvotek™ Mini-Prep Isolation of Plasmid DNA

Includes

• Instructions
• Plasmid LyphoCells™
• Tris Buffer Concentrate
• Sodium Hydroxide Solution
• SDS Solution
• Resuspension Buffer
• Potassium Acetate Solution
• RNase Solution
• UltraSpec-Agarose™
• 10X Gel Loading Solution
• 50X Concentrated Electrophoresis Buffer
• Practice Gel Loading Solution
• FlashBlue™ DNA Stain
• InstaStain™ Blue Cards
• Disposable Pipets
• Microcentrifuge Tubes.

Requires

• Electrophoresis tank
• Power supply
• Variable micropipet and tips
• Balance
• Microwave or hotplate

Introduces the principles of extracting plasmid DNA from bacterial cells.

Small-scale rapid isolation of plasmid DNA is a routine procedure used for screening and analysis of recombinant DNA in cloning and subcloning experiments.

• In this experiment, students isolate plasmid DNA without the use of toxic chemicals like phenol or chloroform
• Complete in 1 hour 45 minutes

Order Info

For 20 plasmid isolations and seven 7x7cm gels.

For Use With (Equipment)

Agarose Gel electrophoresis

For Use With (Application)

Use for screening and analysis of recombinant DNA in cloning and subcloning experiments

Includes

Instructions, plasmid extraction LyphoCells™, Gel-loading solution, UltraSpec-Agarose™ Powder, electrophoresis Buffer, InstaStain™ FlashBlue™ Stain, FlashBlue Plus™