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Edvotek™ Mitochondrial DNA Analysis Using PCR
Advanced-level experiment uses the Polymerase Chain Reaction (PCR) to amplify a specific region of the mitochondrial chromosome and perform gel electrophoresis.
Supplier: Edvotek 332
Includes: Instructions, PCR EdvoBeads™, Mitochondrial Primer Mix Concentrate, EdvoQuick™ DNA Ladder, Control DNA Concentrate, TE Buffer, Proteinase K, UltraSpec-Agarose™, Electrophoresis Buffer (50X), 10X Gel Loading Solution, InstaStain™ Ethidium Bromide, FlashBlue™ Stain, Microcentrifuge & PCR Tubes, Disposable Plastic Cups, Salt Packets, & Wax Beads
Requires :
- Thermal cycler
- Horizontal gel electrophoresis apparatus
- Microcentrifuge
- Hotplate
- Micropipets
- Balance
- Water bath
- UV transilluminator
- 500mL flasks
- All necessary safety equipment
- Distilled or deionized water
Description
- Energy within the cell is produced by the organelles called mitochondria, which have their own DNA (mtDNA)
- Mitochondrial diseases have been mapped to the mtDNA chromosome
- Forensic identification also commonly examines mtDNA
Order Info
Contains materials for 25 individual reactions and six gels.
Specifications
| Instructions, proteinase K, PCR EdvoBeads™, control DNA and primers, microtubes, chelating agent, Agarose, DNA ladder, practice Gel loading solution, Gel loading dye, Buffer, InstaStain™ Ethidium Bromide and FlashBlue™ Stain. Store at -20°C | |
| 1 Kit | |
| Standard PCR | |
| 25 |
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