MilliporeSigma™ Immobilon Western HRP Chemiluminescence Substrates

Premixed, ready-to-use chemiluminescent reagents for detection of HRP-based Westerns

Catalog Number	Mfr. No.	Product Type	Quantity	Sensitivity
WBLUF0500	MilliporeSigma™ WBLUF0500	HRP Substrate	500 mL	∼400 fg
WBLUR0500	MilliporeSigma™ WBLUR0500	HRP Substrate	500 mL	∼1 to 3 pg
WBLUC0500	MilliporeSigma™ WBLUC0500	HRP Substrate	500 mL	∼6 pg

Description

Horseradish peroxidase (HRP) catalyzes the oxidation of luminol in the presence of peroxide, releasing photons (chemiluminescence) that are captured on x-ray films or by CCD cameras. Most HRP substrates are plagued by quickly decaying signal and short shelf life, but Luminata substrates are formulated differently to include stabilizing reagents.

• Consistent results with less pipetting error

The stabilizers added to Luminata substrates:

• Prevent spontaneous oxidation of luminol by peroxide in the absence of HRP
• Enhance the stability of both the luminol and peroxide, ensuring that Luminata substrates are stable for one year in their premixed format
• Stabilize, strengthen, and prolong the chemiluminescence signal once HRP starts to catalyze oxidation

Three different sensitivities for a range of detection needs:

• Immobilon Classico premixed reagent for everyday detection needs
• Immobilon Crescendo premixed reagent for detection in the picogram range
• Immobilon Forte premixed reagent for detection in the femtogram range

This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

Specifications

• Product Type: HRP Substrate
• For Use With (Application): Dot/Slot Blot