Thermo Scientific™ EquiPhi29™ DNA Polymerase

Description

Thermo Scientific EquiPhi29 DNA Polymerase is a proprietary phi29 DNA polymerase mutant developed through in vitro protein evolution.

Thermo Scientific EquiPhi29 DNA Polymerase is a proprietary phi29 DNA polymerase mutant developed through in vitro protein evolution. This enzyme is significantly improved over phi29 DNA polymerase in protein thermostability, reaction speed, product yield, and amplification bias, while retaining all the benefits of the wild-type enzyme.

EquiPhi29 DNA Polymerase possesses strong strand displacement activity and 3'→5' proofreading exonuclease activity that acts preferentially on single-stranded DNA or RNA.

Features of EquiPhi29 DNA Polymerase
• Highest processivity and strand displacement activity—more than 70 kb long DNA stretches can be synthesized
• Low amplification bias
• Extremely high yields of amplified DNA, even from small amounts of template
• Highly accurate DNA synthesis in a short time 

Applications
EquiPhi29 DNA Polymerase may be used in a wide variety of applications including:
• Unbiased whole genome amplification (WGA)
• Rolling circle amplification (RCA)
• Protein-primed DNA amplification
• Cell-free cloning of lethal DNA
• In situ genotyping with padlock probes
• RNA-primed DNA amplification

Notes
1. The addition of pyrophosphatase to the reaction mixture with EquiPhi29 DNA Polymerase may further enhance DNA synthesis
2. Because of the enzyme's 3'→5' proofreading exonuclease, 3’-modified primers are highly recommended.
3. EquiPhi29 DNA Polymerase can amplify DNA in a wide range of temperatures (30–45°C), with an optimal reaction temperature of 42°C.

Specifications

• Product Type: Stand-alone enzyme
• For Use With (Application): MDA-WGA, RCA
• Polymerase: EquiPhi29 DNA Polymerase
• Quantity: 250 U
• Content And Storage:

  • EquiPhi29 DNA Polymerase (25 μL at 10 U/μL)
  • 10X EquiPhi29 DNA Polymerase Reaction Buffer (0.25 mL)
  • 110 mM DTT (0.25 mL)
  
  Store at –15 to –25°C.
  
  

• Optimal Reaction Temperature: 42°C
• Shipping Condition: Dry Ice
• Concentration: 10 U/μL
• Reaction Time: 2 hours

For Research Use Only. Not for use in diagnostic procedures.