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Thermo Scientific™ Exonuclease I (20 U/μL)
GSA_VA

Catalog No. p-4521382 Shop All Thermo Scientific Products
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Degrade single-stranded DNA in a 3'→5' direction with Exonuclease I (ExoI), leaving 5'-terminal dinucleotides intact.

Exonuclease I (ExoI) degrades single-stranded DNA in a 3'≥5' direction. It releases deoxyribonucleoside 5'-monophosphates in a stepwise manner and leaves 5'-terminal dinucleotides intact.

It does not cleave DNA strands with terminal 3'-OH groups blocked by phosphoryl or acetyl groups.

Highlights

  • Active in PCR buffers

Applications

  • Primer removal from PCR mixtures:
    • prior to PCR product sequencing
    • li>for one-tube "megaprimer" PCR mutagenesis
  • Removal of single-stranded DNA containing a 3'-hydroxyl terminus from nucleic acid mixtures
  • Assay for the presence of single-stranded DNA with a 3'-hydroxyl terminus

Note

The enzyme is not suitable for removing 3'-overhangs of dsDNA.

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Specifications

Concentration

20 U/μL

Enzyme

Exonuclease

Quantity

4000 U

Product Type

Exonuclease I

For Research Use Only. Not for use in diagnostic procedures.

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