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Thermo Scientific™ Exonuclease I (20 U/μL)
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Exonuclease I (ExoI) degrades single-stranded DNA in a 3'≥5' direction. It releases deoxyribonucleoside 5'-monophosphates in a stepwise manner and leaves 5'-terminal dinucleotides intact.
It does not cleave DNA strands with terminal 3'-OH groups blocked by phosphoryl or acetyl groups.
Highlights
- Active in PCR buffers
Applications
- Primer removal from PCR mixtures:
- prior to PCR product sequencing li>for one-tube "megaprimer" PCR mutagenesis
Note
The enzyme is not suitable for removing 3'-overhangs of dsDNA.
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Specifications
Concentration
20 U/μL
Enzyme
Exonuclease
Quantity
4000 U
Product Type
Exonuclease I
For Research Use Only. Not for use in diagnostic procedures.
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