Gibco™ Expi293™ Expression Medium

Expi293™ Expression Medium

Expi293™ Expression Medium is a chemically defined, serum-free, protein-free medium for growth and transfection of suspension-adapted human embryonic kidney (HEK) 293 cells. It is specifically designed as a core component to support high density culture of Expi293F™ cells in the Expi293™ Expression System for scalable transient protein expression.

Supports growth of suspension 293 cultures to densities of over 1.5 x 10^7 cells/mL

Transfection compatible serum-free suspension culture medium enables transfection efficiencies of greater than 70% using ExpiFectamine™ transfection reagent

Enables sustained, high level expression of high density transiently transfected cultures, achieving yields of up to 1 gram per liter of recombinant protein

Supports growth and transfection of 293 cells in culture formats of less than 1 mL in multiwell plates to greater than 10L in disposable bioreactors

Does not contain phenol red

Expi293™ Expression Medium is formulated with GlutaMAX™-I reagent. It is ready to use, with no supplementation required. The chemically defined formulation results in high reproducibility and lot-to-lot reliability. Expi293™ Expression Medium contains no human or animal-origin components. The medium is not recommended for adherent cell cultures.

Content And Storage

One 10L Universal Bag
Store in the dark at 2-8°C.

Cell Line

Expi293F™

Classification

Animal Origin-free, Chemically-defined, Protein-free, Serum-free

Form

Liquid

Product Type

Expression Medium

With Additives

GlutaMAX

Without Additives

No Phenol Red

Product Line

Expi293™

Quantity

10 L

For Research use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.