Gibco™ Expi293™ GnTI- Expression System Kit

Expi293 GnTI- Expression System Kit is part of the Expi293 platform for rapid, high-yield transient protein expression from mammalian 293 cells

System kit is centered on engineered Expi293F cells that do not have N-acetylglucosaminyltransferase I (GnTI) activity and therefore lack complex N-glycans. The cell line was established by the selective knockout of the GnTI gene in parental Expi293F cells. This cell line is a powerful tool for the high-yield expression of homogeneously glycosylated recombinant proteins.

Features:

• Homogeneous N-glycosylation of expressed proteins
• Equivalent protein yields to parental Expi293F cells - up to 1 g/L
• Significantly higher protein yields than other HEK293 GnTI- cell line alternatives
• Suspension, high-density culture of Expi293F GnTI- cells in Expi293 Expression Medium
• High-efficiency transient transfection using ExpiFectamine 293 reagent in combination with specialized transfection enhancers

Components included:

• 1 vial of frozen Expi293F GnTI- cells
• 1 L of Expi293 Expression Medium
• 1 ExpiFectamine 293 transfection kit sufficient to transfect 1 L of culture
• 100 mL Opti-Plex Complexation Buffer
• 1 PNGase F Glycan Cleavage kit
• pRABBIT IgG IRES-EmGFP positive control vector

All components of the Expi293 GnTI- Expression System are also available for individual purchase

Delivery Type

Transfection

Quantity

1 kit

Content And Storage

• Expi293F GnTI- Cells (1x10⁷ cells/vial), store in vapor-phase liquid nitrogen
• 1 L Expi293 Expression Medium, store at 2–8°C (protect from light)
• ExpiFectamine 293 Transfection Kit for 1 L of culture, store at 2–8°C
• 100 mL Opti-Plex Complexation Buffer, store at 2–8°C (protect from light)
• PNGase F Glycan Cleavage Kit, store at -20°C
• pRABBIT IgG IRES-EmGFP Positive Control Vector, store at 2–8°C or long term storage at -20°C

Shipping Condition

Approved for shipment at Room Temperature or on Wet or Dry Ice

Cell Line

Expi293F™

Product Line

Expi293™

For Use With (Application)

Protein Production

For Research Use Only. Not for use in diagnostic procedures.