Gibco™ Expi293™ Inducible GnTI- Expression System Kit

Expi293 Inducible GnTI- Expression System Kit is part of the Expi293 platform for rapid, high-yield transient protein expression from mammalian 293 cells

• System kit is centered on engineered Expi293F cells that do not have N-acetylglucosaminyltransferase I (GnTI) activity, therefore lacking complex N-glycans, and stably express the tetracycline repressor (TetR) protein, thus enabling tetracycline-regulated protein expression
• Expi293F Inducible GnTI- cell line was established by the selective knockout of the GnTI gene and the stable integration of the regulatory plasmid pcDNA6/TR, which encodes the TetR gene under the control of the human CMV promoter, in parental Expi293F cells
• This cell line is a powerful tool for tetracycline-inducible high-yield expression of homogeneously glycosylated recombinant proteins.

Features:

• Homogeneous N-glycosylation of expressed proteins
• Tetracycline-inducible protein expression with extremely low basal expression levels
• Equivalent protein yields to parental Expi293F cells—up to 1 g/L
• pcDNA5/TO expression vector for tetracycline-regulated expression of the gene of interest
• Suspension, high-density culture of Expi293F Inducible GnTI- cells in Expi293 Expression Medium
• High-efficiency transient transfection using ExpiFectamine 293 reagent in combination with specialized transfection enhancers

Components included:

• 1 vial of frozen Expi293F Inducible GnTI- cells
• 1 L of Expi293 Expression Medium
• 1 ExpiFectamine 293 transfection kit sufficient to transfect 1 L of culture,
• 100 mL Opti-Plex Complexation Buffer
• 1  PNGase F Glycan Cleavage kit
• 1 pcDNA5/TO Mammalian Expression Vector kit
• 500 mg tetracycline hydrochloride
• pRABBIT IgG IRES-EmGFP positive control vector

All components of the Expi293 Inducible GnTI- Expression System are also available for individual purchase

Delivery Type

Transfection

Quantity

1 kit

Content And Storage

• Expi293F Inducible GnTI- Cells (1x10⁷ cells/vial), store in vapor-phase liquid nitrogen
• 1 L Expi293 Expression Medium, store at 2–8°C (protect from light)
• ExpiFectamine 293 Transfection Kit for 1 L of culture, store at 2–8°C
• PNGase F Glycan Cleavage Kit, store at -20C
• 100 mL Opti-Plex Complexation Buffer, store at 2–8°C (protect from light)
• 20 μg pcDNA5/TO Mammalian Expression Vector, store at 2–8°C or long term storage at -20°C.
• 500 mg Tetracycline Hydrochloride, store at -20°C
• pRABBIT IgG IRES-EmGFP Positive Control Vector, store at 2–8°C or long term storage at -20°C

Shipping Condition

Approved for shipment at Room Temperature or on Wet or Dry Ice

Cell Line

Expi293F™

Product Line

Expi293™

For Use With (Application)

Protein Production

Promoter

CMV/TO

For Research Use Only. Not for use in diagnostic procedures.