Gibco™ Expi293F™ Cells

Expi293F™ human cells are derived from the 293 cell line, and are a core component of the Expi293™ Expression System

• Expi293F™ human cells are derived from the 293 cell line, and are a core component of the Expi293™ Expression System
• They are maintained in suspension culture and will grow to high density in Expi293™ Expression Medium
• Expi293F™ cells are highly transfectable and generate superior protein yields compared to standard 293 cell lines in transient protein expression
• Frozen cells are supplied in a vial containing 1 mL of cells at 1 x 10⁷ viable cells/mL in Expi293™ Expression Medium and 10% DMSO
• The cells should be thawed directly into Expi293™ Expression Medium
• The six-vial multipack eliminates the need to expand and rebank the cells in the lab
• Cells are typically grown in flasks on a shaker platform in a humidified, CO₂ cell culture incubator
• However, cultures are scalable from volumes of 10 L in bioreactors
• Protein harvest can be performed at 2 to 7 days post-transfection, depending on the protein expressed
• For expression of recombinant antibodies, harvest is typically at 5 to 7 days post-transfection
• There is no need for culture medium supplementation or batch feeding during expression

Content And Storage

1 x 10⁷ cells⁄mL, store in liquid nitrogen

Cell Line

Expi293F™

Cell Type

Kidney (Embryonic)

Culture Type

Suspension Cell Culture

Form

Cryopreserved

Species

Human

Media Recommendation

Expi293™ Expression Medium

No. of Cells

1 x 10⁷

Product Line

Expi293™

Product Type

Expi293F Cells

Quantity

1 mL

Shipping Condition

Dry Ice

Source

Human