Gibco™ Expi293F™ Inducible Cells

Expi293F Inducible cell line is derived from engineered Expi293F cells that stably express the tetracycline repressor (TetR) protein, thus enabling tetracycline-regulated protein expression

Cell line established by the stable integration of the regulatory plasmid pcDNA6/TR, which encodes the TetR gene under the control of the human CMV promoter. It is a powerful tool for tetracycline-inducible high-yield expression of recombinant proteins.

Features:

• Suspension, high-density culture in Expi293 Expression Medium
• Tetracycline-inducible protein expression with extremely low basal expression levels
• Equivalent protein yields to parental Expi293F cells—up to 1 g/L
• Significantly higher protein yields than other HEK293 Tet-inducible cell line alternatives

For inducible expression of a protein of interest, pcDNA5/TO mammalian expression vector or an equivalent inducible vector is required for tetracycline-regulated gene expression.

Frozen cells are supplied in a vial containing 1 mL of cells at 1 x 10⁷ viable cells/mL in Expi293 Expression Medium and 10% DMSO. The cells should be thawed directly into Expi293 Expression Medium

Content And Storage

1 vial Expi293F Inducible Cells (1x10⁷ cells/vial), store in vapor-phase liquid nitrogen

Product Line

Expi293™

Quantity

1 vial

Shipping Condition

Dry Ice

For Research Use Only. Not for use in diagnostic procedures.