Gibco™ ExpiCHO™ Expression System Kit

Includes

2 vials frozen ExpiCHO-S™ cells, 1 bottle 1000mL ExpiCHO™ Expression Medium, 1 ExpiFectamine™ CHO transfection kit for 1 L of culture, 1 vial Antibody Expressing Positive Control Vector, 1 bottle 100mL OptiPRO™ SFM; store all components at +2° - +8° C, except frozen cells stored in liquid nitrogen

Provides the highest protein yields possible in a transient CHO system

• Milligram to multigram yields of recombinant protein per liter of culture in 5 to 14 days post-transfection, which are frequently higher than transient 293-based systems
• Cost-effective transient expression in CHO cells, allowing scientists developing protein biotherapeutics to work with CHO-expressed proteins from start to finish in the drug development process
• A robust alternative mammalian transient expression host, allowing for production of proteins that are difficult to express in HEK293 cells
• Flexible, reliable culture and transfection protocols with multiple options that readily fit into current transient expression workflows
• Scalable expression for cultures from <1 mL to >10 L, thus generating the amount of protein needed for your application

Type

Expression System

Protein Tag

Untagged

Delivery Type

Transfection

Content And Storage

• 2 vials frozen ExpiCHO-S cells
• 1000 mL ExpiCHO Expression Medium
• 1 ExpiFectamine CHO Transfection Kit for 1 L of culture
• 1 vial Antibody Expressing Positive Control Vector
• 100 mL OptiPRO SFM

Store all components at 2-8°C, except frozen cells which should be stored in liquid nitrogen.

Shipping Condition

Approved for shipment at Room Temperature or on Wet or Dry Ice

Cell Line

CHO-S, SFM Adapted

Expression System

Mammalian

Product Line

ExpiCHO™

For Use With (Application)

Protein Production, Constitutive Expression

Promoter

CMV

For Research Use Only. Not for use in diagnostic procedures.