Gibco™ ExpiCHO™ Expression System Kit

Description

Includes

2 vials frozen ExpiCHO-S™ cells, 1 bottle 1000mL ExpiCHO™ Expression Medium, 1 ExpiFectamine™ CHO transfection kit for 1 L of culture, 1 vial Antibody Expressing Positive Control Vector, 1 bottle 100mL OptiPRO™ SFM; store all components at +2° - +8° C, except frozen cells stored in liquid nitrogen

Provides the highest protein yields possible in a transient CHO system

• Milligram to multigram yields of recombinant protein per liter of culture in 5 to 14 days post-transfection, which are frequently higher than transient 293-based systems
• Cost-effective transient expression in CHO cells, allowing scientists developing protein biotherapeutics to work with CHO-expressed proteins from start to finish in the drug development process
• A robust alternative mammalian transient expression host, allowing for production of proteins that are difficult to express in HEK293 cells
• Flexible, reliable culture and transfection protocols with multiple options that readily fit into current transient expression workflows
• Scalable expression for cultures from <1 mL to >10 L, thus generating the amount of protein needed for your application

Specifications

• Type: Expression System
• Protein Tag: Untagged
• Delivery Type: Transfection
• Content And Storage: • 2 vials frozen ExpiCHO-S cells
  • 1000 mL ExpiCHO Expression Medium
  • 1 ExpiFectamine CHO Transfection Kit for 1 L of culture
  • 1 vial Antibody Expressing Positive Control Vector
  • 100 mL OptiPRO SFM
  
  Store all components at 2-8°C, except frozen cells which should be stored in liquid nitrogen.
• Shipping Condition: Approved for shipment at Room Temperature or on Wet or Dry Ice
• Cell Line: CHO-S, SFM Adapted
• Expression System: Mammalian
• Product Line: ExpiCHO™
• For Use With (Application): Protein Production, Constitutive Expression
• Promoter: CMV

For Research Use Only. Not for use in diagnostic procedures.