Gibco™ ExpiSf™ Expression System Starter Kit

Gibco™ ExpiSf™ Expression System Starter Kit is a baculovirus-insect cell protein expression system for superior yields and consistent performance run after run using a fast, streamlined workflow.

• A chemically defined, yeastolate-free, animal origin-free medium for consistent cell growth and protein expression
• Integrated components that work in concert to deliver three times higher protein yields compared to traditional insect expression platforms
• Robust production of high-titer, high-quality P0 recombinant baculovirus in suspension culture format without the need for further virus amplification
• A fast, simplified workflow that allows you to obtain protein in as little as 6-10 days; half the time of traditional protocols
• Scalable expression that can be applied to cultures from 24-deep well plates to >3L shake flasks

Type

Insect Cell Protein Expression System

Cloning Method

Restriction Enzyme/MCS

Protein Tag

Untagged

Delivery Type

Baculovirus

Content And Storage

• 1000 mL bottle ExpiSf CD Medium, store in the dark at 2°C to 8°C
• 4 x 1 mL ExpiSf Enhancer, 2°C to 8°C
• 1 mL ExpiFectamine Sf Transfection Reagent, 2°C to 8°C
• 2 x 1 vial ExpiSf9 Cells, store in vapor-phase liquid nitrogen
• 500 mL Opti-MEM, 2°C to 8°C
• 10 μg pFastBac1 vector, 2°C to 8°C
• 4 ng pFastBac1-Gus control vector, 2°C to 8°C
• 5 x 0.1 mL MAX Efficiency DH10Bac Competent Cells, -80°C
• 24 x 125 mL Single-Use Nalgene PETG Erlenmeyer Flasks, store at room temperature

Format

Kit with Erlenmeyer Flasks

Shipping Condition

Approved for shipment at Room Temperature or on Wet or Dry Ice

Cell Line

Sf9, SFM Adapted

Product Line

ExpiSf™

Promoter

Polyhedrin