Gibco™ ExpiSf™ Protein Production Kit

Gibco™ ExpiSf™ Protein Production Kit is designed to significantly boost protein expression from high-density suspension cultures of Sf9 insect cells using the ExpiSf Expression System.

• Delivers greater than 80% more protein yield in high-density suspension Sf9 cell culture than ExpiSf CD Medium alone
• Includes ExpiSf Enhancer, which is specifically designed to work in concert with ExpiSf CD Medium to significantly boost protein expression from baculovirus-infected insect cell cultures
• Demonstrates direct scalability of baculovirus infection from culture volumes of 4mL in deep-well plates to >3 L in shake flasks

The ExpiSf Protein Production Kit is specifically designed to work with ExpiSf9 Cells (Cat. No. A35243) in the ExpiSf Expression System. The kit supplies sufficient ExpiSf Enhancer and ExpiSf CD Medium to infect 1 L, 10 L, or 50 L of culture. ExpiSf CD Medium is also available for purchase separately (Cat. No. A3767802).

Type

Media and reagents, ExpiSf CD Meidum and ExpiSf Enhancer

Quantity

1 L (ExpiSf CD Medium), 4 x 1 mL (ExpiSf Enhancer)

Content And Storage

1000 mL bottle ExpiSf CD Medium, store in the dark at 2°C to 8°C; 4 x 1 mL ExpiSf Enhancer, store (at 2°C to 8°C; Shelf Life: 12 months from date of manufacture)

Classification

Animal Origin-Free, Chemically Defined, Protein-Free, Serum-Free

Compatible Cells

Insect Cells

Format

Liquid

Shipping Condition

Approved for shipment at Room Temperature or on Wet Ice

Expression Mechanism

Cell-Based Expression

Expression System

Baculovirus

Product Line

ExpiSf™