Invitrogen™ EXPRESS One-Step Superscript™ qRT-PCR Kit, universal

Kit incorporates proven Invitrogen™ enzymes specifically tailored to deliver superior results on high-throughput, fast-cycling instruments for probe-based qPCR experiments.

Supplier:  Invitrogen™ 11781200

Catalog No. 11-781-200

Description

• Kit incorporates proven Invitrogen™ enzymes specifically tailored to deliver superior results on high-throughput, fast-cycling instruments for probe-based qPCR experiments
• This fast, one-step mix is designed to work with a variety of instruments’ default protocols, including Applied Biosystems and non-Applied Biosystem (see universal version) qPCR platforms
• Fast-activating, antibody-mediated Platinum Taq DNA Polymerase for complete, rapid activation; provides high sensitivity and reproducibility
• The One-Step SuperScript III Reverse Transcriptase module is a unique formulation to ensure high yield and less PCR inhibition
• Flexible formats with separate or pre-mixed ROX are designed to work with default instrument protocols on a wide range of high-throughput fast instruments
• Contains heat-labile UDG which significantly reduces carryover contamination risk with excellent activity from room temperature to approximately 40°C
• Due to the thermostability of SuperScript III, the cDNA synthesis step is run at 50°C, at which temperature the heat-labile UDG is totally and irreversibly inactivated
• Once thawed, the EXPRESS qPCR SuperMix can be stored for up to 1 month at 4°C to 8°C
• Store at -20°C for long-term storage

Specifications

• Content And Storage: The EXPRESS One-Step SuperScript™ qRT-PCR Universal kit contains 3 modules: 2X EXPRESS SuperScript™ Mix (1 ml), Express qPCR SuperMix Universal (5 ml), and ROX Reference Dye (500 μL).
  
  Store at -20°C for long-term storage. Once thawed, the EXPRESS qPCR SuperMix can be stored for up to 1 month at 4–8°C.
• Format: Tube
• Polymerase: Taq DNA Polymerase
• For Use With (Equipment): 7500 Fast System, 7500 System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, BioRad CFX96™, StepOnePlus™, Fast Mode, StepOnePlus™, Standard Mode, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, BioRad MyiQ, Corbett RotorGene, Cepheid SmartCycler, MJ Opticon, MJ Chromo4, BioRad iCycler iQ
• Product Type: qRT-PCR Kit
• No. of Reactions: 500 Reactions

• Detection Method: Primer-probe
• PCR Method: 1-step RT-qPCR
• Reaction Speed: Fast or Standard
• Product Line: SuperScript™
• Sufficient For: 500 Reactions
• Sample Type: RNA

For Research Use Only. Not for use in diagnostic procedures.