Thermo Scientific™ EZ-Link™ Sulfo-NHS-Biotinylation Kit

Biotinylate protein amines (e.g., lysines) with our shortest (13.5A), water-soluble, NHS-ester biotinylation reagent for antibody or cell-surface labeling.

Label antibodies, proteins and other molecules that have primary amines with Thermo Scientific™ EZ-Link Sulfo-NHS-Biotin, a short-chain, water-soluble biotinylation reagent.

Highlights:

• Shorter spacer arm than Sulfo-NHS-LC-Biotin
• Reacts with primary amines at pH 7-9 in non-amine-containing buffers such as borate, carbonate, or phosphate buffers
• Negative charge of -SO₃ keeps biotinylation localized at the cell surface so reagent does not pass through cell membrane
• Water solubility eliminates the need for harmful organic solvents

U.S. Patents 5,892,628 and 5,872,261.

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Description

EZ-Link Sulfo-NHS-Biotinylation Kit

Product Type

Sulfo-NHS-Biotinylation Kit

Quantity

10 reactions

Content And Storage

Sufficient For: 10 labeling reactions, each with 1 to 10 mg of antibody
• EZ-Link Sulfo-NHS-Biotin, 25 mg
• PBS Pack (makes 500mL), 1 pack
• Zeba Spin Desalting Columns (7K MWCO), 5 mL, 10 columns
• HABA Solution, 1 mL
• Avidin, 10 mg

Store avidin and vial of biotin reagent (with desiccant) at -20°C. Store remaining kit components at 4°C.

Labeling Scale

1 to 10 mg

Product Line

EZ-Link™

Solubility

Water

Label or Dye

Biotin

Labeling Target

Antibodies, Proteins

For Research Use Only. Not for use in diagnostic procedures.