Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin

Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.

Thermo Scientific™ EZ-Link Sulfo-NHS-LC-Biotin is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.

• Protein labeling - biotinylate antibodies to facilitate immobilization, purification or detection using streptavidin resins or probes
• Cell surface labeling - biotinylates only surface proteins of whole cells because the negatively charged reagent does not permeate cell membranes
• Amine-reactive - reacts with primary amines (-NH2), such as lysine side-chains, or the amino-termini of polypeptides
• Soluble - charged sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds
• Irreversible - forms permanent amide bonds; spacer arm cannot be cleaved
• Medium length - spacer arm (total length added to target) is 22.4 angstroms; provides excellent balance between adequate length (minimal steric hindrance for biotin binding) and modest mass

Product Type

Sulfo-NHS-LC-Biotin

Quantity

10 x 1 mg

Content And Storage

Store desiccated at -20°C.

Labeling Method

Chemical Labeling

Shipping Condition

Qualified for Ambient or Wet Ice

Product Line

EZ-Link™

Cell Permeability

Cell-Impermeant

Chemical Reactivity

Amine

Reactive Moiety

NHS Ester

Solubility

DMF (Dimethylformamide), DMSO (Dimethylsulfoxide), Water

Label or Dye

Biotin

Spacer

Mid-length

For Research Use Only. Not for use in diagnostic procedures.