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Thermo Scientific™ EZ-Link™ TFP Ester-PEG12-DBCO

Catalog No. FERC20040 Shop All Thermo Scientific Products
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25 mg

Description

A heterobifunctional crosslinker that contains an amine reactive tetrafluorophenyl (TFP) and a dibenzylcyclooctyne (DBCO) with a water solubilizing long PEG12 spacer in between. Use to label any primary amine containing molecule such as a protein to biomolecule with a distantly attached DBCO group.

  • A heterobifunctional crosslinker that contains an amine reactive tetrafluorophenyl (TFP) and a dibenzylcyclooctyne (DBCO) with a water solubilizing long PEG12 spacer in between the two groups
  • This crosslinker can be used to label any primary amine containing molecule such as a protein to biomolecule with a distantly attached DBCO group
  • Once a protein or (bio)molecule is DBCO labeled, it can undergo a copper-free azide-alkyne cycloaddition reaction to form a stable triazole linkage with an azide labeled coupling partner
  • Can be used to create diverse conjugates with the biorthogonal coupling between an alkyne and azide (supplied separately)
  • The coupling or crosslinking procedure is typically done in two steps:
    • First, a protein or amine containing molecule is labeled with a DBCO group and excess crosslinker is removed
    • Second, an azide containing moiety (prepared independently) is introduced into the same system as the DBCO moiety
    • Labeling occurs under biological conditions without the introduction of any additional reagents such as a copper catalyst
  • Reagent readily dissolves in water-miscible solvents for dilution into aqueous reaction mixtures
  • TFP esters are more hydrolytically stable than NHS esters

Specifications

Chemical Reactivity Amine-Azide
Cleavable No
Description TFP Ester-PEG12-DBCO
Molecular Weight (g/mol) 1067.12
PEGylated Yes
Spacer Arm Length 46.3 Å
Content And Storage Upon receipt store desiccated at -20°C.
Cell Permeability Not determined
Shipping Condition Wet Ice
Product Line EZ-Link™
Labeling Method Chemical Labeling
Crosslinker Type Heterobifunctional
Reactive Moiety TFP Ester, DBCO
Spacer Long (>30 Å)
Form Liquid
Quantity 25 mg
Solubility DMF, DMSO
Format Standard
Water Soluble No
Product Type Crosslinker
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