Thermo Scientific™ DreamTaq DNA Polymerase (5 U/μL)

Thermo Scientific DreamTaq DNA Polymerase is an enhanced Taq DNA polymerase optimized for all standard PCR applications. It ensures higher sensitivity, longer PCR products, and higher yields compared to conventional Taq DNA polymerase.

Catalog Number	Mfr. No.	Color	Quantity
FEREP0701	Thermo Scientific™ EP0701	Colorless	200 Units
FEREP0702	Thermo Scientific™ EP0702	Colorless	500 Units
FEREP0703	Thermo Scientific™ EP0703	Colorless	5 x 500 Units
FEREP0705	Thermo Scientific™ EP0705	Colorless	10 x 500 Units
FEREP0704	Thermo Scientific™ EP0704	Colorless	20 x 500 Units
FEREP0711	Thermo Scientific™ EP0711	Green	200 Units
FEREP0712	Thermo Scientific™ EP0712	Green	500 Units
FEREP0713	Thermo Scientific™ EP0713	Green	5 x 500 Units

Description

Thermo Scientific DreamTaq DNA Polymerase is an enhanced Taq DNA polymerase optimized for all standard PCR applications. It ensures higher sensitivity, longer PCR products and higher yields compared to conventional Taq DNA polymerase. DreamTaq DNA Polymerase uses the same reaction setup and cycling conditions as conventional Taq DNA polymerase. Extensive optimization of reaction conditions is not required. It is supplied with optimized DreamTaq Buffer, which includes 20 mM MgCl₂

DreamTaq Green DNA Polymerase is a combination of DreamTaq DNA Polymerase and 10X DreamTaq Green Buffer. The 10X DreamTaq Green Buffer includes a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with PCR performance and is compatible with downstream applications such as DNA sequencing, ligation, and restriction digestion.

Features

• Robust amplification with minimal optimization
• High yields of PCR products
• Higher sensitivity compared to conventional Taq DNA polymerase
• Amplification of long targets up to 6 kb from genomic DNA and up to 20 kb from viral DNA
• Incorporates modified nucleotides, but does not incorporate dUTP
• Direct loading of PCR product on gel with green format

Applications

• Routine PCR amplification of DNA fragments up to 6 kb
• High throughput PCR
• Genotyping

Notes

• For applications requiring PCR product analysis by absorbance or fluorescence excitation, colorless 10X DreamTaq Buffer (Cat. No. B65) is recommended for purifying PCR products before analysis.

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Specifications

• GC-Rich PCR Performance: Low
• Reaction Speed: Standard
• Fidelity (vs. Taq): 1X
• Overhang: 3'-A

• Polymerase: DreamTaq DNA Polymerase
• Concentration: 5 U/μL
• Hot Start: No

For Research Use Only. Not for use in diagnostic procedures.