Thermo Scientific™ GeneJET Plant RNA Purification Kit

Requires

Dithiothreitol (DTT, #R0861)

GeneJET Plant RNA Purification Mini Kit is designed for rapid and efficient purification of high quality total RNA from wide variety of plant species and tissue types.

GeneJET Plant RNA Purification Mini Kit is designed for rapid and efficient purification of high quality total RNA from wide variety of plant species and tissue types.

The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard procedure takes less than 20 minutes following cell lysis. Purified high quality RNA can be used in a wide range of downstream applications, such as RT-PCR, RT-qPCR, Northern blotting and other RNA-based analysis. RNA yields vary between different species, tissues, and age of tissue sample. See Table 1 for typical total RNA yields from various sources.

Thermo Scientific GeneJET Plant RNA Purification Mini Kit is designed for rapid and efficient purification of high quality total RNA from wide variety of plant species and tissue types.

The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard procedure takes less than 20 minutes following cell lysis. Purified high quality RNA can be used in a wide range of downstream applications, such as RT-PCR, RT-qPCR, Northern blotting and other RNA-based analysis. RNA yields vary between different species, tissues, and age of tissue sample. See Table 1 for typical total RNA yields from various sources.

Highlights
• Fast—procedure completed in 20 min following cell lysis
• Convinient—protocol does not include clogging-prone lysate filtration step
• High yield—up to 65 μg of high purity and high integrity RNA from 50 mg plant sample
• Compatible with wide variety of samples (leaves, roots, sprouts and other parts of various plants)

Applications
Fast extraction of high purity RNA suitable for all conventional molecular biology procedures, including:
• RT-PCR
• RT-qPCR
• Northern blotting
• Nuclease protection assay

Content And Storage

• 40 mL Plant RNA Lysis Solution; store at room temperature
• 40 mL Wash Buffer WB 1 (concentrated); room temperature
• 30 mL Wash Buffer 2 (concentrated); room temperature
• 30 mL Water, Nuclease-free; room temperature
• 50 GeneJET RNA Spin Columns with Collection Tubes; room temperature
• 50 Collection Tubes (2 mL); room temperature
• 50 Collection Tubes (1.5 mL); room temperature

Isolation Technology

Silica Spin Column

Purification Time

20 min. (After sample homogenization)

Elution Volume

50 μL

Sample Type

Plants

Final Product Type

Total RNA

High-throughput Compatibility

Not High-throughput Compatible (Manual)

For Use With (Application)

RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling

Quantity

50 Preps

Scale

Mini

Shipping Condition

Room Temperature

Starting Material Amount

≤100 mg

Yield

Up to 65 μg

For Research Use Only. Not for use in diagnostic procedures.