Thermo Scientific™ TranscriptAid T7 High Yield Transcription Kit

Description

Get high yield in vitro transcription from DNA templates containing a T7 RNA Polymerase promoter, including linearized plasmids or PCR products.

TranscriptAid T7 High Yield Transcription Kit contains reagents for 50 reactions of 20 μL. Each reaction yields up to 200 μg RNA from 1 μg of template in 2 hours (see Figures 1 and 2). The reaction can be scaled up to produce milligram amounts of full-length RNA. The kit can be used to produce both long and short transcripts for applications that require large yields of RNA (see Figure 3). All necessary reagents for transcription are included, as well as the RiboRuler High Range RNA Ladder for sizing and quantification.

Highlights include:

• Exceptionally high yields—up to 200 μg in 2 hours
• Versatile—suitable for both short and long RNA transcripts
• Milligram amounts of RNA in a single, scaled-up reaction
• Flexible—generates unlabeled, labeled or capped RNA
• RiboRuler RNA Ladder supplied with kit for sizing and quantification

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Specifications

• Product Line: TranscriptAid
• Product Type: TranscriptAid T7 High Yield Transcription Kit
• Quantity: 50 reactions

For Research Use Only. Not for use in diagnostic procedures.