Abnova™ FOXO1 Split CISH Probe

FOXO1 Split CISH Probe is designed for the qualitative detection of human FOXO1 gene at 13q14.11 in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).

Supplier:  Abnova™ CS0009

Catalog No. 89-335-546

Description

The probe is intended to be used in combination with the CISH Implementation Kit 2, which provides necessary reagents for specimen pretreatment and post-hybridization processing.

Hybridization signals of digoxigenin-labeled polynucleotides appear dark green distinct dot-shaped (proximal to the FOXO1 breakpoint region), and dinitrophenyl-labeled polynucleotides appear bright red distinct dot-shaped (distal to the FOXO1 breakpoint region.)
Normal situation: In interphases of normal cells or cells without a translocation involving the FOXO1 gene region, two red/green fusion signals appear.
Aberrant situation: One FOXO1 gene region affected by a translocation is indicated by one separate green signal and one separate red signal. Genomic aberrations due to small deletions, duplications or inversions might result in inconspicuous signal patterns. Other signal distribution may be observed in some abnormal samples which might result in a different signal pattern than described above, indicating variant rearrangements.
Unexpected signal patterns should be further investigated.

Specifications

• Content And Storage: The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (18 to 25°C) and mix briefly before use.
• For Use With (Application): CISH-Ce