Cytiva GammaBind Plus Sepharose

Description

Binds to all IgG subclasses. Cytiva GammaBind Plus Sepharose is used for lab-scale affinity purification of IgG from a large variety of species and has better binding to rat and mouse IgG subclasses compared to standard GammaBind Sepharose.

GammaBind Plus Sepharose may be used to analyze and purify classes, subclasses and fragments of immunoglobulins from any biological fluid or cell culture medium.

• Recombinant Protein G type 3 coupled to Sepharose CL-6B for enhanced binding to rat and mouse IgG subclasses at laboratory scale
• Binds to all IgG subclasses from human, mouse, and rat; binds total IgG from guinea pig, rabbit, goat, cow, sheep, and horse
• Yields up to 18mg/ml mouse IgG
• Available in 5ml and 25ml sizes

GammaBind Plus Sepharose is GammaBind G, Type 3, covalently immobilized to Sepharose CL-6B by malimide linkage. GammaBind G, Type 3, a recombinant form of streptococcal protein G, binds to the Fc region of IgG from a variety of mammalian species. Since only the Fc region is involved in binding, the Fab region is still available for binding antigen.

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Specifications

• Flow Rate: >130 cm/h
• Content And Storage: 2°C to 8°C, 20% Ethanol
• Ligand: Recombinant GammaBind G type 3 lacking albumin-binding region
• Particle Size: 90 μm, d_50v is the median particle size of the cumulative volume distribution.
• Quantity: 25 mL