Thermo Scientific™ pCMV-Gaussia-Dura Luc Vector for Luciferase Assays

Description

Drive mutated Gaussia luciferase expression with vectors containing a multiple cloning site (MCS) or control promoter (CMV or TK) for extended signal output.

Thermo Scientific™ pMCSGaussia-Dura Luc Vector is a multiple cloning site plasmid designed to accept a promoter sequence for glow-analysis of gene regulation using the naturally secreting Gaussia luciferase reporter.

The Gaussia Dura Luc Vectors contains a mutant form of the Gaussia luciferase gene that confers better bioluminescent signal stability than native luciferase. Gaussia-Dura luciferase (approx. 20kDa) is a secreted protein, which enables measurement of the reporter activity in media (for real-time assays) and in cell lysates. The pMCS vector contains a multiple cloning site for cloning a promoter to study its regulatory potential. The pCMV and pTK vectors have the luciferase gene under the CMV (Cytomegalovirus) promoter and Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter, respectively. These constitutive expression vectors can be used as normalization controls to account for experimental variation in combination with other reporters.

Highlights:

• Naturally-secreting Gaussia-Dura luciferase gene, optimized for high expression and glow-stability in mammalian systems
• Multiple cloning site (MCS) provides versatility for transfer of regulatory elements from one plasmid to another
• Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
• Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
• High-copy pUC bacterial DNA replication origin
• Two control vectors available with strong (CMV) or weak (TK) constitutive promoters for co-transfection and normalization

Specifications

• Description: pCMV-Gaussia-Dura Luc Vector
• Type: Luc Vector
• Cloning Method: Restriction Enzyme/MCS
• Delivery Type: Transfection
• Quantity: 10 μg
• Content And Storage: Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: -70 to -20°C.
• Reporter Gene: Gaussia Dura Luciferase
• Selection Agent (Eukaryotic): Puromycin
• For Use With (Application): Reporter Assays
• Promoter: CMV

For Research Use Only. Not for use in diagnostic procedures.