Invitrogen™ GeneArt™ pYES1L Vector with Sapphire™ Technology

A ready-to-use, 9.3 Kb linearized BAC/YAC shuttle vector, used for the assembly of DNA fragments with the GeneArt™ High-Order Genetic Assembly System

GeneArt™ pYES1L Vector with Sapphire Technology™ is a ready-to-use, 9.3 Kb linearized BAC⁄YAC shuttle vector, used for the assembly of DNA fragments with the GeneArt™ High-Order Genetic Assembly System (cat#A13285 & A13286). The vector has the capacity to clone up to 110 Kb of DNA fragments. This product only includes the linearized GeneArt™ pYES1L Vector with Sapphire Technology™.

Some of the key features and elements of the GeneArt™ pYES1L Vector with Sapphire Technology™ include:

• TRP1 to allow selection of yeast transformants in tryptophan-deficient medium
• ARS⁄CEN origin to allow stable maintenance of the construct in yeast
• F’ for conjugation
• oriT for maintenance of large constructs in E.coli
• Spectinomycin resistance for selection in E.coli
• 6 Convenient and validated restriction sites flanking the cloning region

For Research Use Only. Not for use in diagnostic procedures.

Antibiotic Resistance Bacterial

Spectinomycin

Cloning Method

Seamless Cloning

Content And Storage

The product contains 3 vials of linear GeneArt pYES1L Vector with Sapphire Technology at a concentration of 50 ng/mL. Vials contain enough plasmid for 10 cloning reactions using the GeneArt High-Order Genetic Assembly System (needs to be purchased separately).

Store the product at -80°C.

The vector is guaranteed stable for 6 months when properly stored.

Sample Type

DNA

For Use With (Application)

Cloning

No. of Reactions

10 Reactions

Number of Fragments

Up to 10 Fragments

Product Line

GeneArt™

Replication Origin

ARS⁄CEN, oriT

Selection Marker Eukaryotic

TRP1

Shipping Condition

Dry Ice

Size

110 kb total (vector plus all inserts)

Vector

pYES1L Linearized

For Research Use Only. Not for use in diagnostic procedures.