Thermo Scientific™ GeneJET RNA Purification Kit

Thermo Scientific GeneJET RNA Purification Kit is a simple and efficient system for purification of total RNA from mammalian cultured cells, tissue, human blood cells, bacteria, and yeast.

Thermo Scientific GeneJET RNA Purification Kit is a simple and efficient system for purification of total RNA from mammalian cultured cells, tissue, human blood cells, bacteria, and yeast.

The kit utilizes a silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious cesium chloride gradients, alcohol precipitation, or toxic phenol-chloroform extractions.

RNA molecules longer than 200 nucleotides can be isolated with the GeneJET RNA Purification Kit in 15 minutes after the lysis step. The high-quality purified RNA can be used in a wide range of downstream applications, including RT-PCR, RT-qPCR, Northern blotting, and other RNA-based analyses.

Highlights
• Universal—can be used for both cell and tissue samples from a wide range of sources: mammalian blood, mammalian cell cultures, mammalian tissues, insect, yeast, and bacteria
• Efficient—high yields of total RNA.
• Fast—15 minutes after lysis step
• Pure—an A260/280 ratio > 1.9
• Convenient—spin columns are capped and assembled with collection tubes

Applications
Fast extraction of high purity RNA suitable for all conventional molecular biology procedures, including:
• RT-PCR
• RT-qPCR
• Northern blotting
• Nuclease protection assay

Includes
Proteinase K Lysis Buffer, Wash Buffer 1 (concentrated), Wash Buffer 2 (concentrated), Nuclease-free Water, GeneJET RNA Purification Columns (pre-assembled with Collection Tubes), Collection Tubes (2 mL), Collection Tubes (1.5 mL), Detailed Protocol

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Content And Storage

• 5 x 600 μL Proteinase K; store at room temperature until opened, -20°C after opening
• 200 mL Lysis Buffer; room temperature
• 200 mL Wash Buffer 1 (concentrated); room temperature
• 100 mL Wash Buffer 2 (concentrated); room temperature
• 125 mL Nuclease-free Water; room temperature
• 250 GeneJET RNA Spin Columns with Collection Tubes; room temperature
• 250 Collection Tubes (2 mL); room temperature
• 250 Collection Tubes (1.5 mL); room temperature

Isolation Technology

Silica Spin Column

Purification Time

15 min. (After sample homogenization)

Elution Volume

50–100 μL

Sample Type

Bacteria, Blood, Cells, Tissue, Yeast, Insects, Enzymatic Reactions

Final Product Type

Total RNA

High-throughput Compatibility

Not High-throughput Compatible (Manual)

For Use With (Application)

RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling

Quantity

250 Preps

Scale

Mini

Shipping Condition

Room Temperature

Starting Material Amount

Bacteria: ≤10⁹ cells
Blood: 0.5 mL
Cells: ≤10⁷
Tissue and Insects: 30 mg
Yeast: ≤4 x 10⁸ cells
Enzymatic reactions: ≤100 μL

Yield

Up to 160 μg

For Research Use Only. Not for use in diagnostic procedures.