Gibco™ Basal Medium Eagle (BME)

Requires

Supplementation

Basal Medium Eagle (BME) is a widely used synthetic basal medium for supporting the growth of many different mammalian cells.

Basal Medium Eagle (BME) is a widely used synthetic basal medium for supporting the growth of many different mammalian cells. BME was originally developed by Harry Eagle for HeLa cells and mouse fibroblasts, when he discovered the minimum requirements for cell growth in vitro. BME has since been used for other human lines, including WI-38 and MRC-5.

This BME is manufactured as follows:

• With: Phenol Red
• Without: L-glutamine, HEPES

The complete formulation is available.

BME contains eight B vitamins, the ten essential amino acids, plus cystine, tyrosine, and glutamine. There are a number of modifications of BME: Minimal Essential Medium, Glasgow's Medium and Dulbecco's Modified Eagle's Medium. This BME formulation contains Earle's salts for use in a CO₂ incubator.

BME contains no proteins, lipids, or growth factors. Therefore, BME requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). BME uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Content And Storage

Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Cell Line

HeLa, mouse fibroblasts, WI-38 and MRC-5

Classification

Animal Origin-free

Concentration

1 X

Form

Liquid

Product Type

Basal Medium Eagle (BME)

Sterility

Sterile-filtered

With Additives

Low Glucose, Phenol Red

Without Additives

No Glutamine, No HEPES, No Sodium Pyruvate

Green Features

Sustainable packaging

Manufacturing Quality

cGMP-compliant under the ISO 13485 standard

Product Line

Gibco™

Quantity

500 mL

Shipping Condition

Room Temperature

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.