Gibco™ DMEM/F-12

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM/F-12 is modified as follows:
With: L-glutamine, Phenol Red
Without: HEPES

The complete formulation is available.

Using DMEM/F-12
DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Content And Storage

Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Cell Line

MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts

Cell Type

Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells

Classification

Animal Origin-free

Concentration

1 X

Form

Liquid

Product Type

DMEM (Dulbecco's Modified Eagle Medium)/F-12

Serum Level

Standard Serum Supplementation

Sterility

Sterile-filtered

With Additives

High Glucose, Glutamine, Phenol Red, Sodium Pyruvate

Without Additives

No HEPES

Green Features

Sustainable packaging

Manufacturing Quality

cGMP-compliant under the ISO 13485 standard

Product Line

Gibco™

Quantity

500 mL

Shipping Condition

Room Temperature

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.