Learn More
Requires
Supplementation
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This DMEM is modified as follows:
With: High Glucose, L-glutamine, HEPES, Phenol Red
Without: Sodium Pyruvate
Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Order Info
Shipping Condition: Room Temperature
Specifications
Content And Storage
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Cell Line
HeLa, 293, Cos-7, and PC-12
Cell Type
Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
Classification
Animal Origin-free
Concentration
1 X
Form
Liquid
Product Type
DMEM (Dulbecco's Modified Eagle Medium)
Serum Level
Standard Serum Supplementation
Sterility
Sterile-filtered
With Additives
High Glucose, Glutamine, HEPES, Phenol Red
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.