Gibco™ DMEM, high glucose, HEPES

Requires

Supplementation

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:
With: High Glucose, L-glutamine, HEPES, Phenol Red
Without: Sodium Pyruvate

Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Content And Storage

Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Cell Line

HeLa, 293, Cos-7, and PC-12

Cell Type

Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells

Classification

Animal Origin-free

Concentration

1 X

Form

Liquid

Product Type

DMEM (Dulbecco's Modified Eagle Medium)

Serum Level

Standard Serum Supplementation

Sterility

Sterile-filtered

With Additives

High Glucose, Glutamine, HEPES, Phenol Red

Without Additives

No Sodium Pyruvate

For Use With (Application)

Mammalian cell culture

Glucose Concentration

4500 mg/L

Green Features

Sustainable packaging

Manufacturing Quality

cGMP-compliant under the ISO 13485 standard

Product Line

Gibco™

Quantity

500 mL

Shipping Condition

Room Temperature

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.