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- Rapid production of milligram to gram quantities of recombinant protein in mammalian cells
- High density cultures of Expi293 cells that result in more expressing cells per milliliter of culture and higher protein yields
- Native folding and mammalian post-translational modifications, such as glycosylation, of expressed proteins
- Easy, robust culture and transfection protocols that readily fit into current transient expression workflows
- Scalable expression that can be applied to cultures from<1mL to >10L, thus generating the amount of protein needed
Specifications
Cell Line
Expi293F™
Content And Storage
2 vials frozen Expi293F cells
1 bottle 1000 mL Expi293 Expression Medium
1 ExpiFectamine 293 transfection kit for 1 L of culture
1 vial Antibody Expressing Positive Control Vector
1 bottle 100 mL Opti-MEM Reduced Serum Medium
Store all components at +2°C to +8°C, except frozen cells are stored in liquid nitrogen
Delivery Type
Transfection
Cell Type
293-derived Cells
Promoter
CMV
Transfection Technique
Lipid-based Transfection
Expression System
Mammalian
Expression Stability
Transient
For Use With (Application)
Protein Expression
Key Functions
Protein Production, Constitutive Expression
For Research Use Only. Not for use in diagnostic procedures.
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