Gibco™ Grace's Insect Medium, unsupplemented, powder

Requires

Requires supplementation with 10% Fetal Bovine Serum (FBS), 3.33g/L lactalbumin hydrolysate and 3.33g/L yeastolate before use as a growth medium as well as ambient CO₂ in order to maintain physiological pH. Powder forms of Gibco cell culture medium require sodium bicarbonate supplementation, pH adjustment and filtration at the time of preparation.

Useful during transfection of insect cells or for protein labeling studies with methionine

Widely used for the growth of Spodeptera frugiperda cells, Sf9 and Sf21. Sf9 and Sf21 cells are routinely used to express recombinant protein using the Baculovirus Expression Vector System (BEVS). Was originally designed for the growth of cells from the Australian Emperor Gum Moth, Antherea eucalypti. It is modified as follows: with L-glutamine, without Lactalbumin hydrolysate, Yeastolate, Sodium bicarbonate. It does not contain lactalbumin hydrolysate and yeastolate commonly found in the supplemented version

Cell Culture, Insect Cell Culture, Sf9 and Sf21 Cell Culture

Order Info

Shipping Condition: Room temperature

Compliance

Manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Content And Storage

Storage conditions: 2-8°C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture

Cell Type

Insect Cell

Form

Powder

Product Type

Grace's Insect Medium

With Additives

Glutamine

Without Additives

No HEPES, No Phenol Red, No Sodium Pyruvate, No Lactalbumin Hydrolysate, No Yeastolate, No Sodium Bicarbonate

Product Line

Gibco™

Quantity

10 x 1 L

Shipping Condition

Room Temperature

For Research Use Only. Not for use in diagnostic procedures.