Gibco™ Ham's F-10 Nutrient Mix, HEPES

Requires

Supplementation

Ham's F-10 Nutrient Mixture was designed for serum-free growth of Chinese hamster ovary (CHO) cells

F-10 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including COS-7, primary rat astrocytes, and rat prostate epithelial cells.

 

• Contains zinc, hypoxanthine, and thymidine
• Serum-free growth of CHO cells in F-10 has led to variety of improved formulations, such as Ham's F-12 Nutrient Mixture (F-12)
• Contains no proteins or growth factors and is modified as follows: with phenol red, HEPES, L-glutamine
• Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
• Uses sodium bicarbonate buffer system (1.2g/L) and therefore requires 5-10% CO₂ environment to maintain physiological pH
• Sustainable packaging

 

Cell Culture, Mammalian Cell Culture

For research use only.

Order Info

Shipping Condition: Room Temperature

Content And Storage

Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Cell Line

CHO, COS-7, and rat prostate epithelial cells

Cell Type

Primary Rat Astrocytes

Classification

Animal Origin-free

Concentration

1 X

Form

Liquid

Product Type

Ham's F-10 Nutrient Mixture

Sterility

Sterile-filtered

With Additives

High Glucose, Glutamine, HEPES, Phenol Red, Sodium Pyruvate

Green Features

Sustainable packaging

Manufacturing Quality

cGMP-compliant under the ISO 13485 standard

Product Line

Gibco™

Quantity

500 mL

Shipping Condition

Room Temperature