Gibco™ Ham's F-10 Nutrient Mix

Requires

Supplementation

Ham's F-10 Nutrient Mixture (F-10) was designed for serum-free growth of Chinese Hamster Ovary (CHO) cells.

Ham's F-10 Nutrient Mixture (F-10) was designed for serum-free growth of Chinese Hamster Ovary (CHO) cells. F-10 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including COS-7, primary rat astrocytes, and rat prostate epithelial cells.

This Ham's F-10 is modified as follows:
With: Phenol Red, L-glutamine
Without: HEPES

The complete formulation is available.

Using Ham's F-10
Compared to other basal media, F-10 contains a wider variety of components, including zinc, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-10 has led to a variety of improved formulations, such as Ham's F-12 Nutrient Mixture (F-12). F-10 contains no proteins or growth factors. Therefore, F-10 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-10 uses a sodium bicarbonate buffer system (1.2 g/L), and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Content And Storage

Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Cell Line

CHO, COS-7, and rat prostate epithelial cells

Cell Type

Primary Rat Astrocytes

Classification

Animal Origin-free

Concentration

1 X

Form

Liquid

Product Type

Ham's F-10 Nutrient Mixture

Sterility

Sterile-filtered

With Additives

High Glucose, Glutamine, Phenol Red, Sodium Pyruvate

Without Additives

No HEPES

Green Features

Sustainable packaging

Manufacturing Quality

cGMP-compliant under the ISO 13485 standard

Product Line

Gibco™

Quantity

500 mL

Shipping Condition

Room Temperature

For Research Use Only. Not for use in diagnostic procedures.