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Requires
Supplementation
Leibovitz's L-15 Medium supports the growth of HEP-2 monkey kidney cells and primary explants of embryonic and adult human tissue. L-15 is buffered by phosphates and free base amino acids instead of sodium bicarbonate. This medium is designed for supporting cell growth in environments without CO2 equilibration.
This L-15 is modified as follows:
With: Galactose, Phenol Red, L-glutamine, Sodium Pyruvate
Without: Glucose, HEPES, Sodium Bicarbonate
The complete formulation is available.
L-15 contains no proteins, lipids, or growth factors. Therefore, F-10 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). Powder forms of Gibco™ L-15 cell culture medium require pH adjustment and filtration at the time of preparation (see protocol for details).
Order Info
Shipping Condition: Room Temperature
Specifications
Content And Storage
Storage conditions: 2-8° C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
Cell Line
HEP-2 monkey kidney cells
Cell Type
Explants of embryonic and adult human tissue
Classification
Animal Origin
Form
Powder
Product Type
Leibovitz's L-15 Medium
Sterility
Sterile-filtered
With Additives
Glutamine, Phenol Red, Sodium Pyruvate, Galactose
Without Additives
No Glucose, No HEPES, No Sodium Bicarbonate
Manufacturing Quality
cGMP-compliant under the ISO 13485 standard
Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. For in vitro diagnostic use.
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