Gibco™ McCoy's 5A (Modified) Medium

Requires

Supplementation

McCoy's 5A (modified) Medium is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues.

McCoy's 5A (modified) Medium is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues. This medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryos, and other tissues.

This McCoy's 5A is modified as follows:
With: High Glucose, L-glutamine, Bacto-peptone, Phenol Red
Without: Sodium Pyruvate, HEPES

The complete formulation is available.

Using McCoy's 5A (modified) Medium
Dr. Thomas McCoy originally formulated McCoy's 5A medium as a modification of Basal Medium 5A. Unlike other media, McCoy's 5A contains the reducing agent glutathione, bacto-peptone, and a high level of glucose. This product also includes Dr. Hsu's addition of Hanks' salts to enable use outside a CO₂ incubator. McCoy's 5A (modified) Medium requires serum supplementation, commonly with 10% Fetal Bovine Serum (FBS). McCoy's 5A (modified) Medium uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Content And Storage

Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Cell Line

Rat fibroblasts

Cell Type

Biopsy Tissues

Classification

Animal Origin

Concentration

1 X

Form

Liquid

Product Type

McCoy's 5A Modified Medium

Sterility

Sterile-filtered

With Additives

High Glucose, Glutamine, Phenol Red, Sodium Bicarbonate

Without Additives

No HEPES, No Sodium Pyruvate

Green Features

Sustainable packaging

Manufacturing Quality

cGMP-compliant under the ISO 13485 standard

Product Line

Gibco™

Quantity

500 mL

Shipping Condition

Room Temperature