Gibco™ McCoy's 5A (Modified) Medium, HEPES

Requires

Supplementation

General-purpose medium that supports propagation of many types of primary cells, established cell lines and explants from biopsy tissues

This medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryos, and other tissues.

 

• Contains reducing agent glutathione, bacto-peptone, and high level of glucose
• It is modified as follows: with high glucose, L-glutamine, bacto-peptone, phenol red, HEPES; without sodium pyruvate.
• Includes Dr. Hsu's addition of Hank's salts to enable use outside CO₂ incubator
• Requires serum supplementation, commonly with 10% Fetal Bovine Serum (FBS)
• Uses a sodium bicarbonate buffer system (2.2g/L) and therefore requires 5-10% CO₂ environment to maintain physiological pH
• Sustainable packaging
• Cell Culture, Mammalian Cell Culture

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

Order Info

Shipping Condition: Room Temperature

Content And Storage

Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Cell Line

Rat fibroblasts

Cell Type

Biopsy Tissues

Classification

Animal Origin

Concentration

1 X

Form

Liquid

Product Type

McCoy's 5A Modified Medium

Sterility

Sterile-filtered

With Additives

High Glucose, Glutamine, HEPES, Phenol Red

Without Additives

No Sodium Pyruvate

Green Features

Sustainable packaging

Manufacturing Quality

cGMP-compliant under the ISO 13485 standard

Product Line

Gibco™

Quantity

500 mL

Shipping Condition

Room Temperature

Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. For human ex vivo tissue and cell culture processing applications.