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Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco™ Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This Medium 199 is modified as follows:
With: L-glutamine, Phenol Red
Without: HEPES
The complete formulation is available.
Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2.
Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Order Info
Shipping Condition: Room Temperature
Specifications
Content And Storage
Storage conditions: 2–8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Cell Line
Rat epithelial
Cell Type
Chick Embryo Fibroblasts, Mouse Pancreatic Epithelium, Rat Lens Tissues
Classification
Animal Origin
Concentration
1X
Form
Liquid
Product Type
Medium 199
Sterility
Sterile-filtered
With Additives
Low Glucose, Glutamine, Phenol Red
Without Additives
No HEPES, No Sodium Pyruvate
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
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