Gibco™ Medium 199, no phenol red

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells.

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco™ Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This M199 is modified as follows:
With: L-glutamine
Without: Phenol Red, HEPES

The complete formulation is available.

Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO₂ incubator, or with Hanks' salts for use without CO₂.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Compliance

Gibco Medium 199 is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Content And Storage

Storage conditions: 2-8° C. Protect from light.
Shipping conditions: Ambient.
Shelf life: 12 months from date of manufacture.

Culture Environment

CO₂

Cell Line

Rat epithelial

Cell Type

Chick Embryo Fibroblasts, Mouse Pancreatic Epithelium, Rat Lens Tissues

Classification

Animal Origin

Concentration

1 X

Form

Liquid

Product Type

Medium 199

Sterility

Sterile-filtered

With Additives

Glutamine, Sodium Bicarbonate

Without Additives

No Phenol Red, No Sodium Pyruvate, No HEPES

Green Features

Sustainable packaging

Manufacturing Quality

cGMP-compliant under the ISO 13485 standard

Osmolality

270 - 310 mOsm/kg

Product Line

Gibco™

Quantity

500 mL

Shipping Condition

Room Temperature

For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.