Gibco™ MEM, powder

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media.

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco™ MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM is modified as follows:
With: L-glutamine, Phenol Red
Without: HEPES, Sodium Bicarbonate

The complete formulation is available.

Gibco™ MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. MEM is available with Earle’s salts for use in a CO₂ incubator, or with Hanks' salts for use without CO₂. This product is made with Earle’s salts.

Using MEM
MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO₂ environment to maintain physiological pH. Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

Order Info

Shipping Condition: Room Temperature

Content And Storage

Storage conditions: 2-8° C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture

Cell Line

HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts

Cell Type

Primary Rat Astrocytes

Classification

Animal Origin-free

Form

Powder

Product Type

MEM (Minimum Essential Medium)

Serum Level

Standard Serum Supplementation

Sterility

Sterile-filtered

With Additives

Low Glucose, Glutamine, Phenol Red

Without Additives

No HEPES, No Sodium Pyruvate, No Sodium Bicarbonate

Manufacturing Quality

cGMP-compliant under the ISO 13485 standard

Product Line

Gibco™

Quantity

10 x 1 L

Shipping Condition

Room Temperature

Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.