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Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco™ MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This MEM is modified as follows:
With: L-glutamine, Gentamycin, Proline, Sodium Pyruvate, Zinc
Without: HEPES, Phenol Red, Insulin
The complete formulation is available.
Gibco™ Richter’s MEM is a modified formulation of Eagle's MEM that contains iron, zinc, putrescine, fatty acids, and gentamycin. Richter’s modification also includes very high concentrations of both choline chloride and i-Inositol. This product is made with Earle’s salts.
Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.
Gibco™ MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Order Info
Shipping Condition: Room Temperature
Compliance
Gibco MEM is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
Specifications
Content And Storage
Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Cell Line
HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Cell Type
Primary Rat Astrocytes
Classification
Animal Origin-free
Concentration
1 X
Form
Liquid
Product Type
MEM (Minimum Essential Medium)
Sterility
Sterile-filtered
With Additives
Glutamine, Sodium Pyruvate, Gentamicin, Proline, Zinc
Without Additives
No HEPES, No Phenol Red, No Insulin
For Research Use Only. Not for use in diagnostic procedures.
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