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Opti-MEM™ Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change to growth rate or morphology. Opti-MEM™ is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM™ can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. We offer a variety of Gibco™ Opti-MEM™ modifications for a range of cell culture applications.
This Opti-MEM™ is modified as follows:
With: L-glutamine, Phenol Red
The complete formulation is confidential. For more information, please contact Technical Services.
Gibco™ Opti-MEM™ is a unique media that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. These additional components allow for a reduction in serum supplementation by at least 50%.
Gibco™ Opti-MEM™ uses a sodium bicarbonate buffer system (2.4 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment and filtration at the time of preparation (see protocol for details).
Order Info
Shipping Condition: Room Temperature
Specifications
Content And Storage
Storage conditions: 2-8° C
Shipping conditions: Ambient
Shelf life: 30 months from date of manufacture
Cell Line
Sp2, AE-1, CHO, BHK-21, and HEK
Cell Type
Primary Fibroblasts
Classification
Animal Origin
Form
Powder
Product Type
Opti-MEM I
Sterility
Sterile-filtered
With Additives
Glutamine, Phenol Red
Manufacturing Quality
cGMP-compliant under the ISO 13485 standard
Product Line
Gibco™, Opti-MEM™
Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. For in vitro diagnostic use.
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