Gibco™ RPMI 1640 Medium, GlutaMAX™ Supplement

Requires

Supplementation

RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium) was originally developed to culture human leukemic cells in suspension and as a monolayer.

RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium) was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This RPMI 1640 is modified as follows:
With: GlutaMAX™, Phenol Red
Without: HEPES
The complete formulation is available.

Using RPMI 1640 Medium
RPMI 1640 medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 medium contains biotin, vitamin B₁₂, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 medium with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. RPMI 1640 medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 medium uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Content And Storage

Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Cell Line

HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas

Cell Type

Leukemic Cells

Classification

Animal Origin-free

Concentration

1 X

Form

Liquid

Product Type

RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)

Serum Level

Standard Serum Supplementation

Sterility

Sterile-filtered

With Additives

GlutaMAX, Phenol Red

Without Additives

No HEPES, No Sodium Pyruvate

Green Features

Sustainable packaging

Manufacturing Quality

cGMP-compliant under the ISO 13485 standard

Product Line

Gibco™, GlutaMAX™

Quantity

500 mL

Shipping Condition

Room Temperature

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.