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Used for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells. TrypLE™ Express cleaves peptide bonds on the C-terminal sides of lysine and arginine, and is a direct replacement for trypsin. Exceptional purity increases specificity and reduces damage to cells that can be caused by other enzymes present in some trypsin extracts.
- Gentle On Cells
- urity increases specificity due to the action of single enzyme, reducing damage caused by cleavage from enzymes present in some trypsin preparations and in other protease extracts
- Room-Temperature Stable for 24 months
- Ready to use
- Can be directly substituted for trypsin in existing protocols
- Dilution alone inactivates TrypLE Express, avoiding the need for trypsin inhibitors, such as FBS
Energy efficient, less hazardous, sustainable packaging
Cell Culture, Mammalian Cell Culture
Order Info
Shipping Condition: Room Temperature
Compliance
Manufactured in facilities compliant with cGMP manufacturing requirements, certified to the ISO 13485 standard, and registered with the FDA as medical device manufacturers.
Specifications
Chelators
EDTA
Content And Storage
Storage conditions: 15°C to 30°C, protect from light
Alternate storage conditions: 2°C to 8°C or -5°C to -20°C
Shipping conditions: Room temperature
Shelf life: 24 months from date of manufacture
Cell Type
Mammalian Cells
Classification
Animal Origin-free
Concentration
1 X
Culture Type
Adherent Cell Culture
Form
Liquid
Product Type
Cell Culture Dissociation Reagent
Sterility
Sterile-filtered
With Additives
Phenol Red, EDTA
For Research Use Only. Not for use in diagnostic procedures.
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